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Compared to a confirmation method using LC-MS/MS, the specificities and sensitivities for both methods were more than 95%. The JWH-250 assay had very limited cross-reactivity, with JWH-250-5-OH-pentyl and JWH-250-5-carboxypentyl metabolites at 50% being the only ones with a cross-reactivity >1%, compared to the JWH-250-4-OH calibrator. The JWH-018 assay had moderate to high cross-reactivity with 17 SC or SC metabolites. (2013) reported two enzyme-linked immunosorbent assays (ELISA) that were designed to detect the 5-OH metabolite of JWH-018 and the 4-OH metabolite of JWH-250 in urine. The antibodies can show cross-reactivity towards other structures than the targeted one, thus expanding the usefulness of the assays, and also enabling the detection of new SC.Īrntson et al. Commercial immunoassay kits have, however, become available for some SC metabolites. 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